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[answered] 1. a) On the figure, write the chemical name for each inter


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1. a) On the figure, write the chemical name for each intermediate in glycolysis.

 

b) Write on the figure the order in which they appear in glycolysis 1st, 2nd, etc.

 

c) List the glycolytic enzyme for which each intermediate is a substrate

 

A is a substrate for the enzyme ___________________

 

B

 

C

 

D

 

E

 

d) Which intermediate shown in the picture is a reactant in substrate level phosphorylation? 2. a) The following exercises are standard questions in most biochemistry courses. Getting the correct answer shows that you have

 

some pretty good understanding of the reactions in glycolysis. I will show you how to work out the problem with the first substrate. 1 Many aspects of glycolysis were elucidated using radiolabeled substrates.

 

The question is: starting with C-14

 

labeled glucose at one specific position

 

(indicated in the formula) predict which

 

carbon (if any) will be radiolabeled in

 

pyruvate, the final product of the

 

pathway.

 

So what we need to do is track the

 

position of C-14 through the different

 

reactions in the pathway.

 

Starting with the C-14 labeled glucose

 

shown in the figure as (B) predict which

 

carbon (if any) will be radiolabeled in

 

pyruvate, the final product of the

 

pathway. c) Same questions starting with glucose C and D. This time draw (no computer generated formulas

 

please) the intermediates yourself (once for each substrate please) and show the final labeled

 

products.

 

Starting with C: Starting with D: 2 3. How does a muscle cell maintain the [NAD+]/[NADH] ratio during the catabolic breakdown of glucose? Examine the aerobic and

 

anaerobic scenarios. Justify your answer. 4. Gluconeogenesis takes place during intense exercise, which seems counterintuitive. Why would an organism synthesize glucose

 

and, at the same time, consume it to generate energy? 5. Which of the carbon(s) of glucose must be labeled with C-14 for the end products of alcoholic fermentation to be ?cold? ethanol

 

(unlabeled ethanol) and 14CO2? Clearly explain your answer. 3 6) Why does the lack of glucose-6-phosphatase activity in the brain and in muscle makes good physiological sense? 7) Compare and contrast the roles of lactate dehydrogenase in gluconeogenesis and in lactic acid fermentation 4

 


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