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[answered] Buffers that match the pH of blood and the cytoplasm are co


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Buffers that match the pH of blood and the cytoplasm are commonly required in scientific experiments. If you were to design a buffer that would have a pH of 7.2, what components would you select?

TR 1. Identify the chemical components and the proportions necessary to achieve a buffer with the desired pH. Include relevant calculations in your answer.


Buffers that match the pH of blood and the cytoplasm are commonly required in scientific experiments. If you were to design a buffer that would have a pH of 7.2, what components would you select?

 

TR 1. Identify the chemical components and the proportions necessary to achieve a buffer with the desired pH. Include relevant calculations in your answer. pH = pka + log (conjugate base / weak acid)

 

This equation is the buffer equation

 

H2PO4-(aq) + H2O(l) HPO42- + H3O+

 

We have the pKa -log(ka) = -log(6.2 x10-8)

 

pH = 7.2

 

a weak acid in conjunction with its base creates a buffer

 

weak acid - H2PO4-(aq)

 

conjugate base - HPO42pH = pKa + log(A-/HA)

 

pH-pKa = log(A-/HA )

 

7.2 - 7.21 = log(HPO42-/ H2PO4-) = -0.01

 

-0.01 (antilog) 10 -0.01 = [HPO42-/ H2PO4 ] = 0.977 mol/L

 

HPO42- = 0.977mol/L

 

Ka = [HPO42- ] [ H3O] / [H2PO4-]

 

[1mol/L]6.2 x 10-8 = [0.977mol/L] [ H3O]

 

6.3 x 10-8 = H3O

 

pH = -log (6.3 x 10-8mol/L) =7.2

 

If the ratio maintained, we will always get a buffer of pH = 7.2

 


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