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LAB TEST OF SALIVARY AMYLASE ON STARCH INSTITUTION NAME TITLE DEPARTMENT Please provide a lab report that explains your procedure, results and conclusions. Remember to include
pictures of the lab set-up and results. Also, I want you to expand this initial experiment by proposing
and testing (in the form of an additional experiment) a testable hypothesis that involves the above
general concept. You will be graded on your use of the scientific method and the creativity/complexity
of your experiment. Both the above experimentation and your original experiment may be included
together into one experiment LAB TEST OF SALIVARY AMYLASE ON STARCH Reagents
1. Benedict's solution
6. source of heat
7. test tubes
8. tube rack PROCEDURE: 1. Fill the 250-mL beaker about 3/4 full of water and place on the hot plate for a boiling water bath.
Keep the water JUST AT BOILING. 2. Mark 3 test tubes A, B and C. "Spit" between 1 and 2 mL of saliva into each test tube. 3. Into tube A, add 2 mL of vinegar. Into tubes B and C, add 2 mL of distilled water. Thump the tubes
to mix. LAB TEST OF SALIVARY AMYLASE ON STARCH 4. Place tube B into the boiling water bath for 5 minutes. After the five minutes, remove from the bath,
and place back into the test tube rack. 5. Add 5 mL of the starch solution to each tube and thump to mix. Allow the tubes to sit for 10 minutes,
occasionally thumping the tubes to mix. 6. Add 5 mL of Benedict's solution to each tube and thump to mix. Place the tubes in the hot water
bath. The reaction takes several minutes to begin. Tube A: Starch + saliva treated with vinegar (acid) Was the test positive or negative?
negative LAB TEST OF SALIVARY AMYLASE ON STARCH
What does this indicate?
The vinegar stops the action of amylase from catalyzing the starch therefore the solution becomes
This indicates that vinegar(acids)distort the shape of proteins in the salivary amylase. Result
The solution turns cloudy. conclusion
acids denature the shape of proteins in the salivary amylase,therefore with the introduction of vinegar
on the starch mixture with amylase results to a negative indication. Tube B: Starch + saliva and water, treated in a boiling water bath Was the test positive or negative?
What does this indicate? LAB TEST OF SALIVARY AMYLASE ON STARCH
It indicates that when enzymes are exposed to extreme temperature for some time ,they are denatured
and therefore become inactive.
the Benedict's solution retained its color as the reaction did not occur.Extreme tamperatures denatures
enzymes,therefore when the salivary amylase was introduced to a hot water bath for five minutes the
amylase was denatured. Conclusion
salivary amylase/enzymes when introduced to hot temperatures it is denatured therefore does not react
on the substrate. Tube C: Starch + saliva Was the test positive or negative?
positive What does this indicate? LAB TEST OF SALIVARY AMYLASE ON STARCH
This indicates that the salivary amylase successfully worked on the starch. Result
the Benedict's solution turned brown as an indication of the presence of sugar.
salivary amylase acts on starch in favorable conditions,i.e is optimum temperature and optimum pH as
in this case.
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